		Technical Data
A0004-02D3	ABCA4 (Retinal-specific ATP-binding Cassette Transporter, ATP-binding Cassette Sub-family A Member 4, RIM ABC Transporter, RIM Protein, RmP, Stargardt Disease Protein, ABCR)

Description:
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-KQQTESHDLPLHPR, from ABCA4, at C-terminal (NP_000341.2).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes ABCA4. Species sequence homology: Canine and human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Singh HP, Jalali S, Hejtmancik JF, Kannabiran C Homozygous null mutations in the ABCA4 gene in two families with autosomal recessive retinal dystrophy Am J Ophthalmol. 2006 May;141(5):906-13.