Technical Data
A0004-02D3
ABCA4 (Retinal-specific ATP-binding Cassette Transporter, ATP-binding Cassette Sub-family A Member 4, RIM ABC Transporter, RIM Protein, RmP, Stargardt Disease Protein, ABCR)
Description:
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-KQQTESHDLPLHPR, from ABCA4, at C-terminal (NP_000341.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes ABCA4. Species sequence homology: Canine and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Singh HP, Jalali S, Hejtmancik JF, Kannabiran C Homozygous null mutations in the ABCA4 gene in two families with autosomal recessive retinal dystrophy Am J Ophthalmol. 2006 May;141(5):906-13.