Technical Data
A0004-02K
ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1, ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD)
Description:
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Applications:
Suitable for use in ELISA and Western Blot. Not suitable for FACS. Other applications not tested.

Recommended Dilution:
Western Blot-1:1,000-1:3,000 (ECL). ABCA1 is known to aggregate. Do not boil samples. Use a reducing buffer: 2X sample buffer): 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl, pH 6.8.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k8B55Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRat
Concentration:
Immunogen:
An A1-N3 peptide (within residues 200-250) of the mouse N-termina ABCA1(chicken egg albumin by means of glutaraldehyde).
Purity:
Ascites
Form
Unpurified mouse ascites, 0.1% sodium azide
Specificity:
Recognizes mouse ABCA1at ~250 kD. It does not react with human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.