Technical Data
A0004-02K1
ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1, ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD) (PE)
Description:
ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.

Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute with sterile 40-50% glycerol, dH2O. Aliquot and store at 4C. Reconstituted product is stable for 6 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10B15Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
ABCA1 transfected HeLa cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Reconstitute with 1ml dH20.
Specificity:
Recognizes murine ABCA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function. Pflugers Arch.453:569-579.