Technical Data
A0004-02Q
ABC A7 (ATP-binding Cassette (ABC) Sub-family A Member 7, Macrophage ABC Transporter, Autoantigen SS-N, ABCA-SSN)
Description:
ABCA7, an ATP-binding cassette, sub-family A (ABC1), member 7, is highly homologous to ABCA1 and mediates cellular cholesterol and phospholipid release by apolipoproteins. The ABCA7 gene is regulated by sterol in the opposite direction to ABCA1 through SRE/SREBP2. The expression of ABCA7 by this regulation is associated with phagocytic activity. As well, it binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells and may mediate cholesterol efflux.

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10,000-1:60,000.
Western Blot: 1:2,000-1:8,000. A 240kD band is seen.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k8J150Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRat
Concentration:
As reported
Immunogen:
Mouse ABCA7 stably-transfected HeLa cells. Epitope: Extracellular determinants on ABCA7 of an unknown position.
Purity:
Ascites
Form
As reported
Specificity:
Specific for ABCA7. Species Crossreactivity: This antibody reacts with mouse ABCA7. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.