Technical Data
A0004-02Y
ABC B5 (ATP Binding Cassette Sub-family B (MDR/TAP) Member 5, ATP-binding Cassette Sub-family B Member 5, ABCB 5, ABCB5, ABCB5alpha, ABCB5beta, EST422562, P-glycoprotein ABCB5)
Description:
ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90kD (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812aa in length. It is possibly a five transmembrane protein with a 247aa N-terminal extracellular domain (ECD) aa1-247 and a 283aa C-terminal cytoplasmic region aa530-812. The ECD contains one ABC transporter aa2-177, while the cytoplasmic region contains a second ABC transporter aa570-808. There are two potential splice forms. One shows a seven aa substitution for aa125-812, while another possesses an alternative start site 445aa upstream of the standard site. Over aa141-247, human ABCB5 shares 64aa identity with mouse ABCB5.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, CQTQHRNTSKKAQ, corresponding to the internal region of ABCB5 (NP_848654.3).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human ABCB5 at ~80kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.