Technical Data
A0004-03A
ABC E1 (ATP-binding Cassette (ABC) Transporter 1)
Description:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Western Blot: 0.3-1ug/ml 70kD band observed in lysates of cell lines A431 (calculated MW of 67.6kDa according to NP_002931.1)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
0.5mg/ml
Immunogen:
Peptide with sequence C-KLNSIKDVEQKK, from the C Terminus of the protein sequence according to NP_002931.1
Purity:
Serum
Form
Supplied as a liquid in Tris-saline, pH 7.3. 0.5% BSA, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Human. Species sequence homology: Epitope is 100% homologous to human, mouse, rat, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.