Technical Data
A0004-04C
ABCG2 (MXR, BCRP, ABCP) (PE)
Description:
ABCG2, also called MXR, BCRP or ABCP, is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane (Rocchi, E. et al., 2000, Biochem. Biophys. Res. Commun. 271:42-46). ABCG2 has also been shown to be expressed at relatively high levels in human hematopoietic stem cells (CD34+, CD38-) and to drop sharply in committed progenitors (CD34+/CD38+) (Scharenberg, C.W. et al., 2002, Blood 99:507-512).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing Bcrp1/ABCG2 on their cell surface within a population and qualitatively determine the density of this receptor on cell surfaces by flow cytometry. Washed cells are incubated with the PE-labeled monoclonal antibody which binds to the cells expressing the Bcrp1/ABCG2 structure. Unbound PE-conjugated antibody is then washed from the cells. Cells expressing Bcrp1/ABCG2 become fluorescently stained, with the intensity of staining directly proportional to the density of the
Bcrp1/ABCG2. Cell surface expression of Bcrp1/ABCG2 is determined by flow cytometric analysis using 488nm wavelength laser excitation.

Storage and Stability:
Maybe stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b9H68Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
~25ug/ml
Immunogen:
Synthetic peptide corresponding to human ABCG2.
Purity:
Purified.
Form
Supplied as a lyophilized powder in 25ug of antibody in 1ml PBS containing 0.1% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human ABCG2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85. 2. Sorrentino, B.P. et al. (1995) Blood 86:491.
3. Pallis, M. and N. Russell (2000) Blood 95:2897. 4. Johnstone, R.W. et al. (1999) Blood 93:1075.
5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665. 6. Zhou, S. et al. (2001) Nat. Medicine 7:1028. 7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660. 8. Bunting, K.D. (2002) Stem Cells 20:11.