Technical Data
A0004-04D
ABCG2 (MXR, BCRP, ABCP) (Biotin)
Description:
ABCG2, also called MXR, BCRP or ABCP, is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane (Rocchi, E. et al., 2000, Biochem. Biophys. Res. Commun. 271:42-46). ABCG2 has also been shown to be expressed at relatively high levels in human hematopoietic stem cells (CD34+, CD38-) and to drop sharply in committed progenitors (CD34+/CD38+) (Scharenberg, C.W. et al., 2002, Blood 99:507-512).

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/million
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9H68Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
3T3 cells that had been transduced a retroviral expression vector containing the human ATP binding cassette transporter G2 (ABCG2) sequence.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with BSA. Reconstitute with 40-50% glycerol, PBS. Conjugated to Biotin
Specificity:
Recognizes human ABCG2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.