Technical Data
A0004-04D1
ABCG2 (MXR, BCRP, ABCP)
Description:
ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-12).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Thr180 of human ABCG2 protein, which lies within the intracellular region of ABCG2 (KLH-coupled).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Specificity:
Recognizes human ABCG2. Species crossreactivity: mouse, rat. Sequence Homology: monkey, bovine, xenopus, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58, 2. Allen, J.D. et al. (1999) Cancer Res 59, 4237-41, 3. Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70, 4. Allikmets, R. et al. (1998) Cancer Res 58, 5337-9, 5. Miyake, K. et al. (1999) Cancer Res 59, 8-13, 6. Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52, 7. Zhou, S. et al. (2001) Nat Med 7, 1028-34., 8. Honscha, W. et al. (2000) Hepatology 31, 1296-304, 9. Scharenberg, C.W. et al. (2002) Blood 99, 507-12, 10. Islam, M.O. et al. (2005) Neurosci Res 52, 75-82, 11. Bunting, K.D. (2002) Stem Cells 20, 11-20, 12. Scharenberg, C.W. et al. (2002) Blood 99, 507-12.