Technical Data
A0004-04M1
ABCG1, Mouse, Control Peptide (ATP-binding Cassette (ABC) Transporter 1)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.

ABCG1 a 678aa protein in human (chr 21q22.3), 666aa in mouse, predominantly expressed in many tissues, in the intracellular compartments mainly associated with endoplasmic reticulum and Golgi complex. It shows 26% sequence homology with ABCG2 protein. ABCG1 protein is involved in macrophage lipid homeostasis, an active member of macrophage lipid export complex.

Source:
14-aa peptide from Mouse ABCG1 conjugated to KLH. ~ C-terminus, Extracellular

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Mouse synthetic peptide
Purity: Purified by HPLC.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity: The mouse control peptide is 100% conserved in rat and 92% in human ABCG1. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity .

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Matthew A. Kennedy et al (2001) JBC, Vol. 276, No: 42, I39438-39447; 2.) Schmitz, G et al (2001), J. Lipid Res. 42 (10), 1513-1520; 3.) Lorkowski, S. et al (2001) Biochem. Biophys. Res Commun. 280 (1), 121-131; 4.) Venkateswaran, A et al (2000) JBC. 275 (19), 14700-14707.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.