Technical Data
A0004-04R1
ABCG2, Human, Control Peptide (ATP-binding Cassette (ABC) Transporter 2)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.

ABCG2 protein has 655aa in human (Chr 4) and 657 in mouse, is an integral membrane protein, which plays a major role in multidrug resistance phenotype of malignant cells, when overexpresssed. It appears in three different forms, containing Arg, Gly or Thr at aa position 482, these variants posses significant differences in their cross-resistance and drug transport patterns. The human ABCG2 protein shows 54% sequence homology with mouse ABCG3 protein.

Source:
14aa peptide from Human ABCG2 conjugated to KLH.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human synthetic peptide
Purity: Purified by HPLC.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity: Human control peptide is 100% conserved in Chimp, and 85% in monkey ABCG2, and only 42% in rat and 28% in mouse ABCG2. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Csilla Ozvegy et al (2002) JBC, Vol. 277, No: 50, 47980-47980; 2.) Lyn Mickley et al (2001) Mammalian Genome 12, 86-88; 3.) L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.