Technical Data
A0004-74
ABIN-1 (A-20 Binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation, HIV-1 Nef-interacting Protein, KIAA0113, Nef-associated Factor 1, Naf1, Nip40-1, TNFAIP3 Interacting Protein 1, TNIP1, Virion-associated Nuclear Shuttling Protein, VAN, hVAN)
Description:
The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-kB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).
ABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7). Overexpression of ABIN-1 inhibits NF-kB activation by a number of stimuli including TNF, IL-1, and LPS. It is widely expressed and can be induced by NF-kB, providing a negative feedback loop of NF-kB signaling. In addition to binding to A20, ABIN-1 can function on other key components of NF-kB signaling including IKK /NEMO (8) and NF-kB family members p100 and p105 (9), and can also inhibit ERK signaling (10). Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11). ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
mmunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the amino terminus of human ABIN-1 protein.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of human total ABIN-1 protein. Species Crossreactivity: mouse, rat. Species sequence homology: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.