Technical Data
A0004-74H
ABIN-1, Recombinant, Human (TNIP1, TNFAIP3 Interacting Protein 1, HIV-1 Nef-interacting Protein, Nef-associated Factor 1, Naf1, Nip40-1, Virion-associated Nuclear Shuttling Protein, VAN, hVAN, KIAA0113, NAF1)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
TNIP1 (TNFAIP3-interacting protein 1) has been shown to interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal. In addition, interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. It may regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.

Source:
Recombinant corresponding to aa94-530 of human TNIP1 protein, fused to His-tag at N-terminus, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSNVTASPTA PACPSDKPAP VQKPPSSGTS SEFEVVTPEE QNSPESSSHA NAMALGPLPR EDGNLMLHLQ RLETTLSVCA EEPDHGQLFT HLGRMALEFN RLASKVHKNE QRTSILQTLC EQLRKENEAL KAKLDKGLEQ RDQAAERLRE ENLELKKLLM SNGNKEGASG RPGSPKMEGT GKKAVAGQQQ ASVTAGKVPE VVALGAAEKK VKMLEQQRSE LLEVNKQWDQ HFRSMKQQYE QKITELRQKL ADLQKQVTDL EAEREQKQRD FDRKLLLAKS KIEMEETDKE QLTAEAKELR QKVKYLQDQL SPLTRQREYQ EKEIQRLNKA LEEALSIQTP PSSPPTAFGS PEGAGALLRK QELVTQNELL KQQVKIFEED FQRERSDRER MNEEKEELKK QVEKLQAQVT LSNAQLKAFK DEEKAREALR QQKRKAKA

Molecular Weight: 51.8kD (458aa) confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
51.8kD
Source: E.coli
Purity: ~75% (SDS-PAGE).
Concentration: ~0.25mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH7.5, 20% glycerol, 5mM DTT, 200mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. He CF., et al. (2010) Lupus 19(10):1181-6. 2. Gateva V., et al. (2009) Nat Genet.41(11):1228-33.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.