Technical Data
A0004-75
ABIN-2 (A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation)
Description:
ABIN-2 binds to the COOH-terminal zinc finger domain of A20. NF-kB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2. The latter also inhibits NF-kB activation induced by overexpression of receptor-interacting protein or TNF receptor-associated factor 2. A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation (ABIN-2) binds to A-20. ABIN-2 interferes with NF-kB activation upstream of the IKK complex and might contribute to NF-kB inhibitory function of A-20.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Mouse heart or lung lysate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptides of human ABIN-2.
Purity:
Purified by Protein G chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human ABIN-2. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. S. Van Huffel, et al.JBC 276(32): 30216-30223 (2001) 2. Klinkenberg M, et al. FEBS Letter 498(1):93-97(2001)