Technical Data
A0005
Abl SH2 Domain
Description:
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation. Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome. This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML.

Western Immunoblot Analysis: Tested by using the antibody (1mg/ml) to detect Abl-Bcr (~210kDa) and c-Abl (~140kDa) in human K562 leukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line.

Immunoprecipitation: Tested by using the antibody (15ug) to immunoprecipitate Abl-Bcr (~210kDa) and c-Abl (~140kDa) from a lysate of human K562 leukemia cells.

Storage and Stability: Stable for 1 year at- 20C from date of shipment. Aliquot to avoid repeated freezing and thawing.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouseRabbit
Concentration:
1mg/ml
Immunogen:
Recombinant protein containing Glutathione S-transferase fused to the SH2 domain of Abl.
Purity:
Protein G purified rabbit IgG
Form
Supplied as 250ug of protein G purified rabbit IgG in 250ul of 0.1M Tris-glycine buffer, pH 7.4.
Specificity:
Recognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Laneuville, P., Semin. Immunol 7: 255266, 1995. 2. Rosenberg, N., Semin. Cancer Biol. 5: 95102, 1994. 3. Wang, J.Y., Curr. Opin. Genet. Dev. 3: 3543, 1993. 4. Cheever, M.A., et al., Ann. N.Y. Acad. Sci. 690: 101112, 1993.