Technical Data
A0005-05
Abl SH3 Domain
Description:
The v-abl oncogene, which was originally described in the murine Abelson retrovirus, encodes a non-receptor tyrosine kinase that can mediate cell transformation (13). Mammalian c-Abl is associated with normal cell growth and differentiation, and is distributed in the nucleus, and the cytoplasm in association with F-actin. In the nucleus, c-Abl has been shown to bind chromatin in a cell cycle regulated manner. Deletion of the F-actin binding and the chromatin binding regions of c-Abl has been shown to block cell growth despite the production of an active tyrosine kinase. In chronic myelogenous leukemia (CML), the c-abl protooncogene translocates from chromosome 9 to the breakpoint cluster region (bcr) on chromosome 22 forming the Philadelphia chromosome (4). This translocation causes the expression of a novel 210kD Abl-Bcr fusion gene product which is only found in malignant cells and is believed to play a crucial role in the pathogenesis of CML.

Applications:
Western Immunoblot Analysis: This lot was tested by using the antibody (1ug/ml) to detect Abl-Bcr (~ 210kDa) and c-Abl (~ 140kDa) in human K562 leukemia cells and NFC murine erythroleukemia cells. Note: The amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj and Hut lymphoid cell lines, or murine Wehi myeloid cell line.

Immunoprecipitation: 15ug immunoprecipitates Abl-Bcr (~210kDa) and c-Abl (~ 140kDa) from a lysate of human K562 leukemia cells.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (2550%) and freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
1mg/ml
Immunogen:
Recombinant protein containing Glutathione S-transferase fused to the SH3 domain of Abl-amino acids 9291020.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a solution in 0.1M Tris-glycine buffer, pH 7.4.
Specificity:
Recognizes human p210 bcr-abl , p140 c-Abl , as well as viral p160 gag-abl.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Laneuville, P., Semin. Immunol 7: 255266, 1995. 2. Rosenberg, N., Semin. Cancer Biol. 5: 95102, 1994. 3. Wang, J.Y., Curr. Opin. Genet. Dev. 3: 3543, 1993. 4. Cheever, M.A., et. al., Ann. N.Y. Acad. Sci. 690: 101112, 1993.