Technical Data
A0007-05
Abl Kinase Substrate (Biotin) (Abltide)
1mg
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Technical Data |
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A0007-05 |
Abl Kinase Substrate (Biotin) (Abltide) |
1mg |
| Molecular Biology | Storage: -20°CShipping: RT |
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Synthetic (linear) peptide (EAIYAAPFAKKK), biotinylated at the C-terminus. Substrate for Abl kinase. (with an extra K conjugated to Biotin) Application: Suitable for use in Abl kinase assays. Other applications not tested. Recommended Dilution: Use at 25-50uM final concentration for in vitro Abl kinase assays. 25uM was phosphorylated using 10-20ng of Abl, active. The substrate was captured using Streptavidin, agarose conjugate. Assay results show a 332-fold increase in phosphate transferred to the peptide over background. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. Molecular Weight: 1563D |
Purity: ~ 90%, as determined by HPLC Concentration: ~1mg/ml Form: Supplied as a lyophilized powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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