Technical Data
Abl Kinase Substrate (Abltide)
Molecular Biology Storage: -20CShipping: Blue Ice
Synthetic linear peptide (EAIYAAPFAKKK)(K); substrate for Abl kinase.

Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. Abl contains 407 amino acids (residues 237-643 of the p120-gag-abl polyprotein) which include the kinase catalytic domain, SH2 domain on the N-terminus and the I237M mutation. Abl is identical to the normal c-Abl. Abl is autophosphorylated on tyrosine(s).

Suitable for use in Kinase assay. Other applications not tested.

Recommended Dilution:
Kinase Assay: This lot at 50uM, was successfully phosphorylated using Abl, active. Assay background was subtracted from the actual counts. Representative results from a previous lot are
shown at right. This peptide may also be used as a substrate for Abl (T315I), active and Abl, active, mouse.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C.
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Source: Synthetic peptide
Purity: ~90% determined by HPLC and mass spectroscopy.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Songyang, Z., et al., Nature 373: 536-539, 1995.
General References: 1. Reddy, P.E. et al. (1983) Proc. Natl. Acad. Sci. USA, 80, 3623-3627. 2. Foulkes, J.G. et al. (1985) J. Biol. Chem., 260, 8070-8077. 3. Oppi, C. et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 8200-8204.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.