Technical Data
A Broth (Powder)
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Ammonium Sulfate1
Potassium Phosphate Dibasic10.5
Potassium Phosphate Mono4.5
Sodium Citrate0.5
Total g/L:16.5g
500g makes 32 liters
A Broth is a minimal medium base used in the maintenance and propagation of E.coli.

Directions per Liter: Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White to off-white, homogeneous, free flowing powder Solubility (16.5g/L)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).