Technical Data
A0193-03
ACADL, Recombinant, Human, aa31-430, His-tag (Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, LCAD)
50ug
250ug
Molecular Biology Storage: -20CShipping: Blue Ice
ACADL belongs to the acyl-CoA dehydrogenase family, existing as a homotetramer. It is involved in the catabolism of fatty acids and aa and they provide a major source of energy for the heart and skeletal muscle. Defect in the gene encoding ACADL leads to non-ketotic hypoglycemia and hypotonia, muscle weakness.

Source:
Recombinant corresponding to aa31-430 from human ACADL, fused to His-tag at N-terminal expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGGEERLETP SAKKLTDIGI RRIFSPEHDI FRKSVRKFFQ EEVIPHHSEW EKAGEVSREV WEKAGKQGLL GVNIAEHLGG IGGDLYSAAI VWEEQAYSNC SGPGFSIHSG IVMSYITNHG SEEQIKHFIP QMTAGKCIGA IAMTEPGAGS DLQGIKTNAK KDGSDWILNG SKVFISNGSL SDVVIVVAVT NHEAPSPAHG ISLFLVENGM KGFIKGRKLH KMGLKAQDTA ELFFEDIRLP ASALLGEENK GFYYIMKELP QERLLIADVA ISASEFMFEE TRNYVKQRKA FGKTVAHLQT VQHKLAELKT HICVTRAFVD NCLQLHEAKR LDSATACMAK YWASELQNSV AYDCVQLHGG WGYMWEYPIA KAYVDARVQP IYGGTNEIMK ELIAREIVFD K

Molecular Weight:
~46.7kD (MALDI-TOF)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~46.7kD
Source: E.coli
Purity: ~85% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT, 0.15M sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Maher AC., et al. (2010) Mol Genet Metab. 100(2):163-7. 2. Wanders RJ., et al. (1998) Biochim Biophys Acta. 1393(1):35-40.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.