Technical Data
A0193-15D
ACADM (Medium-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, MCAD)
Description:
ACADM is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.01-0.1ug/ml
Immunohistochemistry (paraffin): 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Liver and Heart lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-RLIVAREHIDKYKN, from C-terminus of the protein sequence according to NP_000007.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human ACADM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K.Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.Proc Natl Acad Sci USA. 1986 Sep;83(17):6543-7. PMID: 3462713.