Technical Data
A0194
ACADS, aa25-412, Recombinant Human, His-Tag (Acyl-Coenzyme A dehydrogenase, SCAD, ACAD3
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency). Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Source:
Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli

AA sequence:
MGSSHHHHHH SSGLVPRGSH MLHTIYQSVE LPETHQMLLQ TCRDFAEKEL FPIAAQVDKE HLFPAAQVKK MGGLGLLAMD VPEELGGAGL DYLAYAIAME EISRGCASTG VIMSVNNSLY LGPILKFGSK EQKQAWVTPF TSGDKIGCFA LSEPGNGSDA GAASTTARAE GDSWVLNGTK
AWITNAWEAS AAVVFASTDR ALQNKSISAF LVPMPTPGLT LGKKEDKLGI RGSSTANLIF EDCRIPKDSI LGEPGMGFKI AMQTLDMGRI GIASQALGIA QTALDCAVNY AENRMAFGAP LTKLQVIQFK LADMALALES ARLLTWRAAM LKDNKKPFIK EAAMAKLAAS EAATAISHQA IQILGGMGYV TEMPAERHYR DARITEIYEG TSEIQRLVIA GHLLRSYRS

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
44.0 kDa
Source: E.coli
Purity: ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 0.1M sodium chloride, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.McAndrew RP., et al. (2008) J Biol Chem. 283(14):9435-43.
2.Tafti M., et al. (2003) Nat Genet. 34(3):320-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.