Technical Data
ACAD8 (Acyl-Coenzyme A Dehydrogenase 8, Mitochondrial) Recombinant, Human
Molecular Biology Storage: -20CShipping: Blue Ice
Acyl-coenzyme A (CoA) dehydrogenases (ACADs) are a family of mitochondrial enzymes that catalyze the first dehydrogenation step in the bets-oxidation of fatty acyl-CoA derivatives. Several human ACADs exist and all ACADs catalyze the same initial dehydrogenation of the substrate at the beta-carbon atom and require electron transfer flavoprotein as an alectron acceptor. The predicted 415-amino acid ACAD8 protein contains many of the residues conserved in most other ACADs, including an active site glutamic acid residue and residues important for tetramer formation.

Recombinant Human ACAD-8 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-415 and having a total molecular mass of 47.7kD. Recombinant Human ACAD8 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

ELISA MS Inhibition Assays Western Blotting

rhACAD8 although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% sodium azide, 10mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Sudden death in a young woman from medium chain acyl-coenzyme A dehydrogenase (MCAD) deficiency. J Emerg Med 2006 Apr;30(3):291-4 2. Suppression of estrogen-related receptor alpha and medium-chain acyl-coenzyme A dehydrogenase in the acute-phase response. J Lipid Res 2005 Oct;46(10):2282-8 3. L-carnitine and exercise tolerance in medium-chain acyl-coenzyme A dehydrogenase (MCAD) deficiency: a pilot study. J Inherit Metab Dis 2005;28(2):141-52 4. Functional interference between estrogen-related receptor alpha and peroxisome proliferator-activated receptor alpha/9-cis-retinoic acid receptor alpha heterodimer complex in the nuclear receptor response element-1 of the medium chain acyl-coenzyme A dehydrogenase gene. J Mol Endocrinol 2003 Aug;31(1):47-60 5. Very-long-chain acyl-coenzyme a dehydrogenase deficiency in mice. Circ Res 2003 Sep 5;93(5):448-55 6. Comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme A dehydrogenase-novel analog complexes. Arch Biochem Biophys 2003 Jan 15;409(2):251-61

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.