Technical Data
A0196-01D
ACSL5 (Long-chain-fatty-acid-CoA Ligase 5, Long-chain acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250)
Description:
ACSL5 is an isozyme of the long chain fatty acid coenzyme A ligase family. All isozymes of this family convert free long chain fatty acids into fatty acyl CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. ACSL5 is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid induced glioma cell growth. Three transcript variants encoding two different isoforms have been found.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: >1:16,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Spleen lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-RTQIDSLYEHIQD, from the C-terminus of the protein sequence according to NP_057318.2; NP_976313.1; NP_976314.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human ACSL5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N. Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5. Int J Colorectal Dis. 2005 Apr 5; [Epub ahead of print] PMID: 15809837.