Technical Data
A0196-01E
ACSL5, CT (Long-chain-fatty-acid-CoA Ligase 5, Long-chain Acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250)
Description:
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:16,000
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-RTQIDSLYEHIQD from the C-terminal of human ACSL5.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human ACSL5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.