		Technical Data
A0219-90	ACAT1, CT (Acetyl-CoA Acetyltransferase, Mitochondrial, Acetoacetyl-CoA Thiolase, T2, ACAT, MAT)

Description:
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within residues 305-335 in the C-terminal region of human ACAT1.

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human ACAT1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Locke,J.A.,Prostate 68(1), 20-33 (2008). 2.Guo,Z.Y.,Biochemistry 46(35), 10063-10071 (2007). 3.Haapalainen,A.M.,Biochemistry 46(14), 4305-4321 (2007).