		Technical Data
A0220-01J	AcCoA Acetyltransferase (ACAT, Acetoacetyl CoA Thiolase, Acetyl CoA Acetyltransferase Mitochondrial, Acetyl Coenzyme A Acetyltransferase 1, ACAT1, MAT, RATACAL, T2, THIL)

Description:
Acely-CoA:cholestorl acyltransferase (ACAT) is an integral membrane enzyme that belongs to type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of
intracellular cholesterol homeostasis at single cell level. Two isoform of ACAT exists, ACAT1 and ACAT2, are expressed in liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzehimer’s Diease, condition caused by excess amount of amyloid beta-peptide (Abeta).
Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to human ACAT.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Specificity:

Recognizes human ACAT. Species Crossreactivity: Mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Buhman KF et al. Biochim Biophys Acta 1529(1-3): 142-54, 2000 2. Puglielli L et al. Nat Cell Biol 3(10) 905-12, 2001. 3. Chang C et al. Acta Biochim Biophys Sin 38(3): 151-156, 2006.