Technical Data
A0220-02
ACAT1 (Acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1)
Description:
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:2000
Western Blot: 0.1-0.3ug/ml ~45kD band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kD according to Human NP_000010.1 and 44.81kD to Mouse NP_659033.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-DEEYKRVDFSKVPK, from the internal region of human ACAT1 (NP_000010.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human ACAT1. Species crossreactivity: mouse, rat. Species sequence homology: canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2): E474-82.