Technical Data
A0224
Acetyl CoA Carboxylase (ACC1)
Description:
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:25
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate buffer is recommended.
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul -20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase a1 conjugated to KLH. MW of Antigen: 280kD.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity: bovine, mouse, rat and monkey. Species Sequence Homology: chicken and Drosophila 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jianqiang M, et al. PNAS 100:7515-7520, 2003 2. Girad J, et al. The FASEB Journal 8:36-42, 1994 3. Wakil SJ, et al. Annu Rev Biochem 52:537579, 1983 4. Abu-Elheiga L, et al. Science 291: 2613-2616. 200 5. Ruderman, N.B. et al., Am. J. Physiol. 276, E1E18, 1999 6. Ha, J. et al., J. Biol. Chem. 269, 2216222168, 1994 7. Abu-Elheiga, L. et al., Science 291, 26132616, 2001 8. Levert, K.L. et al,. J. Biol. Chem. 277, 1634716350, 2002