Technical Data
A0225
AcCoA Carboxylase (Ser79), phosphorylated (ACC, Acetyl Coenzyme A Carboxylase)
Description:
Acetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi- subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500 detects phosphorylated AcCoA Carboxylase in lysate from lambda phosphatase-treated and untretated A375 cells.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic linear peptide corresponding to aa73-85, C-HMRSSM[pS]GLHLVK, from rat AcCoA carboxylase conjugated to KLH (NP_942131). Species Sequence Homology: bovine, canine, chicken, rat and mouse
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes AcCoA Carboxylase phosphorylated at Ser79, Mr 257kD. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kemp, B.E., et al., Trends Biochem. Sci. 24: 22-25 (1999). 2. Chen, Z.P., et al., Am. J. Physiol. Endocrinol. Metab. 279: E1202-E1206 (2000). 3. Andersson, U., et al., J. Biol. Chem. 279: 12,005-12,008 (2004). 4. Chan, A.Y., et al., J. Biol. Chem. 279: 32,771-32,779 (2004). 5. Miyazaki, M., et al., J. Biol. Chem. 279: 35,017-35,024 (2004). 6. Orci, L., et al., al. (2004). Proc Natl Acad Sci U.S.A. 101: 2058-63. 7. Nagata, D., et al., al. (2003). J Biol Chem. 278: 31000-6.