Technical Data
Acetyl-CoA BioAssay™ Kit
Kits and Assays Storage: -20°CShipping: Blue Ice
Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates.

Acetyl-CoA BioAssay™ Kit is a highly sensitive assay for determining Acetyl CoA levels in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe® to generate fluorescence (Ex/Em=535/587nm).

The assay can detect 10 to 1000pmol of Acetyl CoA (with detection limit ~0.4uM) in a variety of samples.

Kit Components:
A0225-02A: Acetyl CoA Assay Buffer, 1x25ml
A0225-02B: PicoProbe®, 1x200ul
A0225-02C: Conversion Enzyme, 1x100ul
A0225-02D: Acetyl CoA Enzyme Mix, 1x500ul
A0225-02E: Acetyl CoA Substrate Mix, 1x220ul
A0225-02F: CoA Quencher, 1x1ml
A0225-02G: Quench Remover, 1x220ul
A0225-02H: Acetyl CoA Standard (1umol), 1x1 vial

Storage and Stability:
Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature
before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.