Technical Data
AcCoA Carboxylase, Phosphorylated (Ser79) (ACC, Acetyl Coenzyme A Carboxylase)
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland, while the 280kD ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at serine 79, or by PKA at serine 1200, inhibits the enzymatic activity of ACC. Recent research indicates that ACC is a potential target of antiobesity drugs.

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:600, with heating in citrate buffer used as antigen retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20°CBlue IceRatRabbit
Not Determined
Synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC.
Purified by Protein A and immunoaffinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes endogenous levels of rat Ser79 phosphorylated ACC. Recognizes both ACCalpha and ACCbeta in humans. MW of Antigen: 280kD. Species Crossreactivity: mouse, human and monkey. Species Sequence Homology: chicken and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ruderman, N.B., et al., Am. J. Physiol. 276: E1-E18 (1999). 2. Ha, J., et al., J. Biol. Chem. 269: 22,162-22,168 (1994). 3. Abu-Elheiga, L., et al., Science 291: 2613-2616 (2001). 4. Levert, K.L., et al., J. Biol. Chem. 277: 16,347-16,350 (2002).