		Technical Data
A0225-30B	AcCoA Synthase 2, 3-hydroxy-3-methylglutaryl, Mitochondrial (HMGCS2, Coenzyme A Synthase 2)

Description:
HMGCS2 is an enzyme that condenses acetyl CoA with acetoacetyl CoA to form HMG CoA, which is the substrate for HMG CoA reductase. Together with HMG CoA lyase, it is responsible for ketone body biosynthesis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Pab Rb x Ch
I1906-12: IgY, Chicken (FITC) Pab Rb x Ch
I1906-14: IgY, Chicken (AP) Pab Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgY		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice		Chicken

Concentration:

~0.5mg/ml

Immunogen:

Protein domain corresponding to aa271-487 of HMGCS2. Cellular Localization: Mitochondrial.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes AcCoA Synthase 2. Species Crossreactivity: human, mouse, and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bouchard, L., et al., Pediatr. Res. 49(3): 326-331 (2001). 2. Boukaftane, Y. and Mitchell, G.A., et al., Gene 195(2): 121-126 (1997). 3. Mascaro, C., et al., Arch. Biochem. Biophys. 317(2): 385-390 (1995). 4. Boukaftane, Y., et al., Genomics 23(3): 552-559 (1994). 5. Strausberg, R.L., et al., PNAS 99(26): 16,899-16,903 (2002).