		Technical Data
A0526-35C	Acetylcholine Receptor, alpha (CHRNA1, ACHRA, CHNRA, Cholinergic receptor, nicotinic, alpha 1 (muscle), ACHRD, CMS2A, FCCMS, SCCMS)

Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: Neat
Immunohistochemistry (Frozen): 1:3
Immunoprecipitation: 1:100
Optimal working dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H508	Ascites

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human Acetylcholine Receptor (AChR)

Purity:

Ascites

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human AChR-alpha subunit, exhibiting homogenous and high avidity binding to the receptor. It is a good Pan AChR marker as it binds well to normal, denervated and fetal AChR and shows strong binding to endplate AChRs. Useful for studying the autoimmune response in myasthenia gravis and the maturation of the human AChR during development. Species Crossreactivity: Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Whiting, P.J., et al., J. Neuroimmunol. 11: 223-235 (1986). 2. Roberts, A., et al., Autoimmunity 21: 53-60 (1992). 3. Heidenreich, F., et al., Autoimmunity 2: 31-37 (1988).