Technical Data
A0526-36A
Acetylcholine Receptor, beta (CHRNB1, ACHRB, CHRNB, Cholinergic receptor, nicotinic, beta 1 (muscle), CMS1D, CMS2A, SCCMS, FLJ57107)
Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: Neat. Use 5ul of antibody to label 10e6 cells.
Immunohistochemistry (frozen): 1:3
Immunoprecipitation: 1:100
Western Blot: 1:100
Optimal working dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H510Ascites
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human Acetylcholine Receptor (AChR).
Purity:
Diluted ascites.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human AChR beta subunit. Exhibits homogenous, high avidity binding to the receptor. Binding to recombinant beta subunit demonstrated on Western Blot. Species Crossreactivity: Does not react with mouse, rat or chicken Acetylcholine Receptor, beta.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Whiting, P.J., et al., J. Neuroimmunol. 11: 223235 (1986). 2. Roberts, A., et al., Autoimmunity 21: 5360 (1992). 3. Heidenreich, F., Autoimmunity 2: 3137 (1988). 4. Whiting, P., et al., Neurology 36: 612617 (1986). 5. Schluep, M., et al., Annals of Neurology 22: 212222 (1987).