		Technical Data
A0526-36A	Acetylcholine Receptor, beta (CHRNB1, ACHRB, CHRNB, Cholinergic receptor, nicotinic, beta 1 (muscle), CMS1D, CMS2A, SCCMS, FLJ57107)

Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: Neat. Use 5ul of antibody to label 10e6 cells.
Immunohistochemistry (frozen): 1:3
Immunoprecipitation: 1:100
Western Blot: 1:100
Optimal working dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3H510	Ascites

Size		Storage	Shipping	Source	Host
500ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human Acetylcholine Receptor (AChR).

Purity:

Diluted ascites.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human AChR beta subunit. Exhibits homogenous, high avidity binding to the receptor. Binding to recombinant beta subunit demonstrated on Western Blot. Species Crossreactivity: Does not react with mouse, rat or chicken Acetylcholine Receptor, beta.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Whiting, P.J., et al., J. Neuroimmunol. 11: 223–235 (1986). 2. Roberts, A., et al., Autoimmunity 21: 53–60 (1992). 3. Heidenreich, F., Autoimmunity 2: 31–37 (1988). 4. Whiting, P., et al., Neurology 36: 612–617 (1986). 5. Schluep, M., et al., Annals of Neurology 22: 212–222 (1987).