Technical Data
ACHRB (CHRNB1, Acetylcholine Receptor Subunit beta, CHRNB)
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1-3ug/ml detects a band of ~55kD in human brain (cerebellum, hippocampus, frontal cortex) lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-QEQEDHDALKED, from an internal region of human CHRNB1 (NP_000738.2). Species sequence homology: bovine, mouse, rat
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Recognizes human CHRNB1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lou XY, Ma JZ, Payne TJ, Beuten J, Crew KM, Li MD. Gene-based analysis suggests association of the nicotinic acetylcholine receptor beta1 subunit (CHRNB1) and M1 muscarinic acetylcholine receptor (CHRM1) with vulnerability for nicotine dependence. Hum Genet. 2006 Oct;120(3):381-9. Epub 2006 Jul 28.