Technical Data
Acetylcholine Receptor (Muscarinic 1)
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that mediates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m1 receptor is localized primarily in brain tissue.

Suitable for use in Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 2ug/ml. Detects an ~64kD protein representing recombinant mouse m1AChR.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ug-20CBlue IceRabbit
Fusion protein corresponding to aa269-320 from the human Acetylcholine Receptor (Muscarinic 1). Species Sequence Homology: mouse, rat and bovine
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Recognizes the human Acetylcholine Receptor (Muscarinic 1). Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ladner, C.J. & Lee, J.M., Exp. Neurol. 158: 451-458 (1999). 2. Wang, P., et al., J. Pharmacol. Exp. Ther. 273: 959-966 (1995).