Technical Data
Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)
Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Suitable for use in ELISA, Immunohistochemistry and Immunoprecipitation. Not suitable for Western Blot and FACS.
Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:50. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul4C (-20C Glycerol)Blue IceHumanMouse
Not determined
Purified human cerebellar acetylcholinesterase. Cellular location: cell membrane and secreted.
Supplied as a liquid in PBS with 0.05% sodium azide.
Recognizes human Acetylcholinesterase. Does not detect butyrylcholinesterase (BChE). Cannot be used in Western blot to detect AChE. Species Crossreactivity: rabbit, bovine, feline and guinea pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.General: This antibody has been used in: Cellular and Molecular Neurobiology, 8(1): 85-93, 1988. 2. General:Brain Research, 539: 233-238, 1991.J. Neural. Transm., 34: 139-1450, 1991.