Technical Data
A0526-43
Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)
Description:
Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k2E7Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Bovine G4-acetylcholinesterase, purified and detergent solubilized (1).
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Recognizes Acetylcholinesterase (bovine brain, AChE)Has specificity for native, heat denatured and SDS plus heat denatured AChE. Reacts with AChE from bovine and human brain or erythrocytes. No cross-reactivity to BtChE from human serum, AChE from flounder body muscle or Torpedo marmorata.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060. 2. Massoulie J, Bon S (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106. 3. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453. 4. Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340. 5. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153.