Description:
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD (1,2).
Applications:
Suitable for use in ELISA, Erythroadsorption Immunoassay (EAIA), Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1,k | 12B4 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ul | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Brain acetylcholinesterase, human, purified and detergent solubilized (1). |
| Purity: |
| Purified by Protein A/G affinity chromatography from culture supernatant. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide. |
| Specificity: |
| Has specificity for human acetylcholinesterase. No cross-reaction is seen with human BtChE, or with AChE from flounder or Torpedo marmorata. Reacts with both G2-and G4-AChE (native detergent soluble) in ELISA and EAIA using the antibody as capture antibody. Reacts 17 times stronger with human brain AChE than with human erythrocyte AChE. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060. 2. Massoulie J, Bon S (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106. 3. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453. 4. Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340. 5. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153. 6. Norgaard-Pedersen B, Hangaard J, Bjerrum OJ (1983) Quantitative enzyme antigen immunoassay of acetylcholinesterase in amniotic fluid. Clin Chem 29:1061-1064.
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