Technical Data
Acid Phosphatase, Human (Biotin)
Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
1ml-20CBlue IceHumanRabbit
Not determined
Acid phosphatase isolated and purified from human seminal plasma.
Purified by ammonium sulphate precipitation and ion exchange chromatography.
Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Recognizes Acid phosphatase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
reactivity Cross-reactivities against enzymes of other sources may occur but have not been determined.
characteristics IgG protein concentration 10 mg/ml. No foreign proteins added.
Intended use This product is intended for use in precipitating and non-precipitating antibody-binding assays
(such as e.g., ELISA and Western blotting and immunofluorescence or histochemical
techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of
the customers own choice.
Directions for use Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may
be between 1/1,000 and 1/10,000.
Handling The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4C;
prolonged storage at or below -20C. It is reconstituted by adding 1.0 ml sterile distilled water,
spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or
below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down
again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS,
pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be
stored at +4C, not refrozen, and preferably used the same day. If a slight precipitation occurs
upon storage, this should be removed by centrifugation. It will not affect the performance of
the product.
Packing Vial with 10 mg IgG (7S) fraction, lyophilised from phosphate buffered saline.
Storage / shelf life Lyophilised at +4C
reconstituted at or below -20C
reconstituted at +4C
at least 10 years
3-5 years
7 days
Caution This product should be handled by qualified persons only and appropriate precautions should
be taken in its handling and disposal, and of all associated materials. For in vitro laboratory
research purposes only.

P.O. Box 22, 5000 AA Tilburg, The Netherlands
Tel. +31 13 535 10 85, Fax: +31 13 536 45 89
Nordic Immunological Laboratories 2004 Quality Control Department