		Technical Data
A0570-05	Acid Sensitive Ion Channel 1 (ASIC1, BNAC 2, Amiloride-sensitive brain sodium channel, ACCN2)

Description:
ASIC1 is a member of a family of Na +channels that are activated by external protons. The family includes another three members ASIC2, ASIC3 and ASIC4. The ASICs are in fact part of a larger superfamily termed epithelial Na +channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (Rat brain membranes): 1:200.
Immunohistochemistry: Rat brain sections
Optimal dilutions to be determined by the researcher.

Control Antigen:
A0570-05A

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Rat	Rabbit

Concentration:

~0.8mg/ml

Immunogen:

Synthetic peptide CQKEAKRSSADKGVALSLDD corresponding to aa469-488 of rat ASIC1 (accession number P55926 ). Epitope location: Intracellular, C-terminus.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide before the addition of glycerol to 40%.

Specificity:

Recognizes rat ASIC1a and ASIC1b. Species Sequence Homology: human: identical; mouse: 19/20 residues identical.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kellenberger, S., et al., Physiol. Rev. 82: 735 (2002). 2. Wemmie, J. A. et al. (2002) Neuron 34, 463. 3. Wemmie, J. A. et al. (2003) J. Neurosci. 23, 5496.