		Technical Data
A0574-02B	Acid Sensitive Ion Channel 2b (ASIC2B, MDEG2 Mammalian Degenerin2)

Description:
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:300-1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa15-26, CSGPGRFMAREQ of human ASIC 2B.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes human Acid Sensitive Ion Channel 2b. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lingueglia, E., et al. (1997) J. Biol. Chem. 272: 29778-29783. 2. Waldmann, R., et al. (1999) Ann. NY Acad. Sci. 869: 67-75.