Technical Data
A0576-03
Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC)
Description:
Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (perfusion fixed tissue): 1:50 Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose, PBS.
Immunocytochemistry: 1:50 ASIC3 transfected cells and primary neuronal cell cultures.
Optimal dilutions to be determined by the researcher.

Additional Information:
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer
(pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4C for 18-24 hours with antibody. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatGuinea pig
Concentration:
Not determined
Immunogen:
Synthetic peptide (C-TASLDPDDFDPEPSDPLGSP) residues 285-304 from the extracellular domain of rat ASIC3.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes rat ASIC3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.