		Technical Data
A0578-79	Acidic Hair Keratin, K33 (hHa3)

Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not Determined

Immunogen:

Synthetic peptide corresponding to EKP IGS CVT NPC GPR SR of human hair (trichocytic) keratin K33 (hHa3) (KLH).

Purity:

Serum

Form

Supplied as a liquid in 0.09% sodium azide.

Specificity:

Recognizes human hair keratin K33 (hHa3) expressed starting from the middle hair cortex. Because of high sequence homology both keratin isoforms K33a (hHa3-I) and K33b (hHa3-II) are detected. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.