Technical Data
A0578-79
Acidic Hair Keratin, K33 (hHa3)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to EKP IGS CVT NPC GPR SR of human hair (trichocytic) keratin K33 (hHa3) (KLH).
Purity:
Serum
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K33 (hHa3) expressed starting from the middle hair cortex. Because of high sequence homology both keratin isoforms K33a (hHa3-I) and K33b (hHa3-II) are detected. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.