Technical Data
A0578-79
Acidic Hair Keratin, K33 (hHa3)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step. Incubate for 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human hair (trichocytic) keratin K33, conjugated to KLH (Formally designated keratin hHa3; EKP IGS CVT NPC GPR SR).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide
Specificity:
Recognizes human hair keratin K33 (hHa3) expressed starting from the middle hair cortex. Because of high sequence homology both keratin isoforms K33a (hHa3-I) and K33b (hHa3-II) are detected. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein, L., Rogers, M., Winter, H., Prätzel, S. & Schweizer, J., J. Biol. Chem. 276: 35,123-35,132 (2001). 2. Langbein, L., Spring, H., Rogers, M.A., Praetzel, S. & Schweizer, J., Methods in Cell Biology 78: 413-456 (2004).