		Technical Data
A0578-81	Acidic Hair Keratin, K34 (KRT34, Keratin 34, HA4, Ha-4, Hair Keratin, Type I Ha4, hHa4, HHA4, HKA4, K34, Keratin, Type I Cuticular Ha4, Keratin-34, KRTHA4 hHa4)

Description:
Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, it is recommended to preincubate the fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to GPCGTSQKGCCN of human acidic hair (trichocytic) keratin K34 (hHa4) (KLH).

Purity:

Serum

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human hair keratin K34 (hHa4) expressed in the upper hair cortex cells. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Langbein, L., et al., J. Biol. Chem. 274: 19,874-19,893 (1999). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).