Technical Data
A0578-81
Acidic Hair Keratin, K34 (KRT34, Keratin 34, HA4, Ha-4, Hair Keratin, Type I Ha4, hHa4, HHA4, HKA4, K34, Keratin, Type I Cuticular Ha4, Keratin-34, KRTHA4 hHa4)
Description:
Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, it is recommended to preincubate the fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to GPCGTSQKGCCN of human acidic hair (trichocytic) keratin K34 (hHa4) (KLH).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K34 (hHa4) expressed in the upper hair cortex cells. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein, L., et al., J. Biol. Chem. 274: 19,874-19,893 (1999). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).