		Technical Data
A0578-85	Acidic Hair Keratin, K36 (KRT36, Keratin 36, HA6, Hair keratin, type I Ha6, hHa6, HHA6, HKA6, K36, Keratin, type I cuticular Ha6, Keratin-36, KRTHA6, hHa6)

Description:
Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. For enhancement of cortex preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.
Western Blot (ECL): 1:2000 Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not Determined

Immunogen:

Synthetic peptide corresponding to human acidic hair (trichocytic) keratin K36, conjugated to KLH. (Formally designated keratin hHa6; APQ VGT QIR TIT EEI C)

Purity:

Serum

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human hair keratin K36 expressed starting from the middle region of cortex.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Langbein L, Rogers M, Winter H, Prätzel S, Beckhaus U, Rackwitz HR, Schweizer J: The catalog of human hair keratins: I. Expres- sion of nine type I members in the hair follicle. J Biol Chem 274, 19874-19893 (1999) 2. Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J: Hair keratins and hair follicle-specific epithelial keratins. Methods in Cell Biology 78, 413-456 (2004)