		Technical Data
A0578-89	Acidic Hair Keratin, K38 (KRT38, Keratin 38 HA8, Hair keratin, type I Ha8, hHa8, HHA8, HKA8, K38, Keratin, type I cuticular Ha8, Keratin-38, KRTHA8, hHa8)

Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen sections): 1:100. For enhanced cortex staining, preincubate fixed sections with 0.1% Triton X-100 in PBS for 1-5 min prior to first antibody incubation step. Suggested incubation time: 1hour at RT.
Western Blot: 1:1000 by ECL
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Guinea pig

Concentration:

Not determined

Immunogen:

Synthetic peptide (TTCGPTCGASTTGSRF) of human acidic hair (trichocytic) keratin hHa8 (KLH).

Purity:

Serum

Form

Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes specifically human hair keratin hHa8 expressed starting from the middle cortex. Staining in the middle cortex appears highly heterogeneous. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Langein L, et al. 1999. J. Biol. Chem. 274:19874-19893. 2. Langbein L, et al. 2004. Methods in Cell Biology. 78:413-456.