Technical Data
ACK1, phosphorylated (Tyr518) (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2) (AP)
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47aa C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
PabIgGAffinity Purified
200ul4C Do Not FreezeBlue IceHumanRabbit
As reported
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr518 of human ACK1 (KLH).
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
Recognizes human ACK1 when phosphorylated at Tyr518.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Guo A, et al. (2008) A 105, 692-7. 2.Rikova K, et al. (2007) Cell 131, 1190-203. 3.Huang PH, et al. (2007) Proc Natl Acad Sci USA 104, 12867-72. 4.Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci USA 104, 5860-5. 5.Goss VL, et al. (2006) Blood 107, 4888-97. 6.Wolf-Yadlin A, et al. (2006) Mol Syst Biol 2, 54. 7.Rush J, et al. (2005) Nat Biotechnol 23, 94-101.