Technical Data
A0650-01C-AP
ACK1, phosphorylated (Tyr518) (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2) (AP)
Description:
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47aa C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr518 of human ACK1 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.
Specificity:
Recognizes human ACK1 when phosphorylated at Tyr518.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Guo A, et al. (2008) A 105, 692-7. 2.Rikova K, et al. (2007) Cell 131, 1190-203. 3.Huang PH, et al. (2007) Proc Natl Acad Sci USA 104, 12867-72. 4.Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci USA 104, 5860-5. 5.Goss VL, et al. (2006) Blood 107, 4888-97. 6.Wolf-Yadlin A, et al. (2006) Mol Syst Biol 2, 54. 7.Rush J, et al. (2005) Nat Biotechnol 23, 94-101.