		Technical Data
A0650-01E	ACK1, CT (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2)

Description:
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47aa C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within residues 939-969 in the C-terminal region of human ACK1.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human ACK1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003). 2. Manser, E., et al., Nature 363(6427):364-367 (1993).